Product Info Summary
SKU: | A03719 |
---|---|
Size: | 100μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-Rab 18 Antibody
SKU/Catalog Number
A03719
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Rab 18 Antibody catalog # A03719. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Rab 18 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03719)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
5A6
Isotype
IgG
Immunogen
Synthesized peptide derived from the Internal region of human Rab 18. at AA rangle: 80-160
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A03719 is reactive to Rab18 in Human, Mouse, Rat
Applications
A03719 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
23.035kDa
Background of Rab18
CAND1 is also known as TIP120A, and TATA-binding protein-interacting protein 120A. The SCF complex consists of the invariable components Skp1, Cul1, and Rbx1 as well as a variable F-box protein, and functions as an E3 ubiquitin ligase. E3 ubiquitin ligases regulate various physiological processes. CAND1 binds to Cul1 and potentially regulates the SCF complex. CAND1 physically associates with Cul1 in the nucleus and this interaction is mediated by a central region of Cul1 distinct from its binding sites for Skp1 and Rbx1. CAND1 selectively binds to unneddylated CUL1 and is dissociated by CUL1 neddylation. CAND1 forms a ternary complex with CUL1 and ROC1.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
Click image to see more details
Western Blot (WB) analysis of MCF-7 cells using Rab 18 Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For Rab18 (Source: Uniprot.org, NCBI)
Gene Name
Rab18
Full Name
Ras-related protein Rab-18
Weight
23.035kDa
Superfamily
small GTPase superfamily
Alternative Names
RAB18 small GTPase; RAB18, member RAS oncogene family; RAB18LI1; ras-related protein Rab-18 Rab18|AA959686|RAB18, member RAS oncogene family|ras-related protein Rab-18|GTP-binding protein Rab18
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Rab18, check out the Rab18 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Rab18: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Rab 18 Antibody (A03719)
Hello CJ!
No publications found for A03719
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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6 Customer Q&As for Anti-Rab 18 Antibody
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for adrenal gland using anti-Rab 18 antibody A03719. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-04-10
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-10
Question
Is this A03719 anti-Rab 18 antibody reactive to the isotypes of RAB18?
Verified Customer
Verified customer
Asked: 2020-03-20
Answer
The immunogen of A03719 anti-Rab 18 antibody is Synthesized peptide derived from the Internal region of human Rab 18. at AA rangle: 80-160. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-03-20
Question
Will anti-Rab 18 antibody A03719 work on primate WB with corpus callosum?
Verified Customer
Verified customer
Asked: 2019-10-24
Answer
Our lab technicians have not validated anti-Rab 18 antibody A03719 on primate. You can run a BLAST between primate and the immunogen sequence of anti-Rab 18 antibody A03719 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate corpus callosum in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-24
Question
Would A03719 anti-Rab 18 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-02-21
Answer
It shows on the product datasheet, A03719 anti-Rab 18 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-02-21
Question
Do you have a BSA free version of anti-Rab 18 antibody A03719 available?
C. Li
Verified customer
Asked: 2018-06-19
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Rab 18 antibody A03719 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-06-19
Question
Is a blocking peptide available for product anti-Rab 18 antibody (A03719)?
S. Li
Verified customer
Asked: 2016-11-23
Answer
We do provide the blocking peptide for product anti-Rab 18 antibody (A03719). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2016-11-23